backbone vector prk5 Search Results


agei sali digested prk5 vector backbone  (New England Biolabs)
97
New England Biolabs agei sali digested prk5 vector backbone
Agei Sali Digested Prk5 Vector Backbone, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 97 stars, based on 1 article reviews
agei sali digested prk5 vector backbone - by Bioz Stars, 2026-03
97/100 stars
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prk5 vector  (Addgene inc)
96
Addgene inc prk5 vector
Prk5 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
prk5 vector - by Bioz Stars, 2026-03
96/100 stars
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prk5 empty vector  (Addgene inc)
90
Addgene inc prk5 empty vector
Wnts affect ALDH1A2 expression in WiT49 cells. (A) Effects of Wnts on TCF reporter activity in WiT49 cells. 400 ng TOPFlash or FOPFlash constructs with 100 ng <t>pRK5-mWnts</t> expression vectors or pRK-5 empty vector, as well as 25 ng of the pRL-TK renilla control vector, was transiently transfected into WiT49 cells for 48 h. The pRK5-mWnts include pRK5-mWnt1, pRK5-mWnt2b, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt5a, pRK5-mWnt6, pRK5-mWnt7a, pRK5-mWnt7b, pRK5-mWnt9b, and pRK5-mWnt11. Luciferase reporter activities relative to renilla (pRL-TK) are shown. Values are the mean ± SD of four determinations; representative results from at least three separate experiments are shown. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 compared with their respective controls, calculated using the Student’s t -test. (B,C) Effect of Wnts on ALDH1A2 expression in WiT49 cells. WiT49 cells were transiently transfected with pRK5-mWnt1, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt9b, or the empty vector pRK5 for 48 h. The ALDH1A2 expression relative to TBP was measured by real-time RT-PCR (B) . Values are the mean ± SD of three determinations; representative results from at least three separate experiments. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 compared with the empty vector pRK5 control group. The ALDH1A2 protein expression was measured by Western blot (C) . pRM-67-ALDH1A2, positive control of ALDH1A2 protein made by transfection of mammalian expression vector pReceiver-M67-ALDH1A2 to WiT49 cells. pRM-67, negative control of ALDH1A2 protein generated by transfection of pReceiver-M67 empty vector. Per lane, 50 μg of the sample was loaded, and 2 μg ALDH1A2 protein-positive control was loaded. Mock represents samples of cells treated with transfection reagent and without plasmid DNA. Values under bands were calculated by the density of bands of ALDH1A2 divided by the density of bands of GAPDH.
Prk5 Empty Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
prk5 empty vector - by Bioz Stars, 2026-03
90/100 stars
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sgrna backbone vector  (Addgene inc)
97
Addgene inc sgrna backbone vector
Wnts affect ALDH1A2 expression in WiT49 cells. (A) Effects of Wnts on TCF reporter activity in WiT49 cells. 400 ng TOPFlash or FOPFlash constructs with 100 ng <t>pRK5-mWnts</t> expression vectors or pRK-5 empty vector, as well as 25 ng of the pRL-TK renilla control vector, was transiently transfected into WiT49 cells for 48 h. The pRK5-mWnts include pRK5-mWnt1, pRK5-mWnt2b, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt5a, pRK5-mWnt6, pRK5-mWnt7a, pRK5-mWnt7b, pRK5-mWnt9b, and pRK5-mWnt11. Luciferase reporter activities relative to renilla (pRL-TK) are shown. Values are the mean ± SD of four determinations; representative results from at least three separate experiments are shown. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 compared with their respective controls, calculated using the Student’s t -test. (B,C) Effect of Wnts on ALDH1A2 expression in WiT49 cells. WiT49 cells were transiently transfected with pRK5-mWnt1, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt9b, or the empty vector pRK5 for 48 h. The ALDH1A2 expression relative to TBP was measured by real-time RT-PCR (B) . Values are the mean ± SD of three determinations; representative results from at least three separate experiments. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 compared with the empty vector pRK5 control group. The ALDH1A2 protein expression was measured by Western blot (C) . pRM-67-ALDH1A2, positive control of ALDH1A2 protein made by transfection of mammalian expression vector pReceiver-M67-ALDH1A2 to WiT49 cells. pRM-67, negative control of ALDH1A2 protein generated by transfection of pReceiver-M67 empty vector. Per lane, 50 μg of the sample was loaded, and 2 μg ALDH1A2 protein-positive control was loaded. Mock represents samples of cells treated with transfection reagent and without plasmid DNA. Values under bands were calculated by the density of bands of ALDH1A2 divided by the density of bands of GAPDH.
Sgrna Backbone Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 97 stars, based on 1 article reviews
sgrna backbone vector - by Bioz Stars, 2026-03
97/100 stars
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prk5 mwnt11  (Addgene inc)
90
Addgene inc prk5 mwnt11
Wnts affect ALDH1A2 expression in WiT49 cells. (A) Effects of Wnts on TCF reporter activity in WiT49 cells. 400 ng TOPFlash or FOPFlash constructs with 100 ng <t>pRK5-mWnts</t> expression vectors or pRK-5 empty vector, as well as 25 ng of the pRL-TK renilla control vector, was transiently transfected into WiT49 cells for 48 h. The pRK5-mWnts include pRK5-mWnt1, pRK5-mWnt2b, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt5a, pRK5-mWnt6, pRK5-mWnt7a, pRK5-mWnt7b, pRK5-mWnt9b, and pRK5-mWnt11. Luciferase reporter activities relative to renilla (pRL-TK) are shown. Values are the mean ± SD of four determinations; representative results from at least three separate experiments are shown. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 compared with their respective controls, calculated using the Student’s t -test. (B,C) Effect of Wnts on ALDH1A2 expression in WiT49 cells. WiT49 cells were transiently transfected with pRK5-mWnt1, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt9b, or the empty vector pRK5 for 48 h. The ALDH1A2 expression relative to TBP was measured by real-time RT-PCR (B) . Values are the mean ± SD of three determinations; representative results from at least three separate experiments. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 compared with the empty vector pRK5 control group. The ALDH1A2 protein expression was measured by Western blot (C) . pRM-67-ALDH1A2, positive control of ALDH1A2 protein made by transfection of mammalian expression vector pReceiver-M67-ALDH1A2 to WiT49 cells. pRM-67, negative control of ALDH1A2 protein generated by transfection of pReceiver-M67 empty vector. Per lane, 50 μg of the sample was loaded, and 2 μg ALDH1A2 protein-positive control was loaded. Mock represents samples of cells treated with transfection reagent and without plasmid DNA. Values under bands were calculated by the density of bands of ALDH1A2 divided by the density of bands of GAPDH.
Prk5 Mwnt11, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prk5 mwnt11/product/Addgene inc
Average 90 stars, based on 1 article reviews
prk5 mwnt11 - by Bioz Stars, 2026-03
90/100 stars
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prk5 mwnt3a  (Addgene inc)
91
Addgene inc prk5 mwnt3a
Wnts affect ALDH1A2 expression in WiT49 cells. (A) Effects of Wnts on TCF reporter activity in WiT49 cells. 400 ng TOPFlash or FOPFlash constructs with 100 ng <t>pRK5-mWnts</t> expression vectors or pRK-5 empty vector, as well as 25 ng of the pRL-TK renilla control vector, was transiently transfected into WiT49 cells for 48 h. The pRK5-mWnts include pRK5-mWnt1, pRK5-mWnt2b, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt5a, pRK5-mWnt6, pRK5-mWnt7a, pRK5-mWnt7b, pRK5-mWnt9b, and pRK5-mWnt11. Luciferase reporter activities relative to renilla (pRL-TK) are shown. Values are the mean ± SD of four determinations; representative results from at least three separate experiments are shown. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 compared with their respective controls, calculated using the Student’s t -test. (B,C) Effect of Wnts on ALDH1A2 expression in WiT49 cells. WiT49 cells were transiently transfected with pRK5-mWnt1, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt9b, or the empty vector pRK5 for 48 h. The ALDH1A2 expression relative to TBP was measured by real-time RT-PCR (B) . Values are the mean ± SD of three determinations; representative results from at least three separate experiments. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 compared with the empty vector pRK5 control group. The ALDH1A2 protein expression was measured by Western blot (C) . pRM-67-ALDH1A2, positive control of ALDH1A2 protein made by transfection of mammalian expression vector pReceiver-M67-ALDH1A2 to WiT49 cells. pRM-67, negative control of ALDH1A2 protein generated by transfection of pReceiver-M67 empty vector. Per lane, 50 μg of the sample was loaded, and 2 μg ALDH1A2 protein-positive control was loaded. Mock represents samples of cells treated with transfection reagent and without plasmid DNA. Values under bands were calculated by the density of bands of ALDH1A2 divided by the density of bands of GAPDH.
Prk5 Mwnt3a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prk5 mwnt3a/product/Addgene inc
Average 91 stars, based on 1 article reviews
prk5 mwnt3a - by Bioz Stars, 2026-03
91/100 stars
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prk5 mwnt4  (Addgene inc)
91
Addgene inc prk5 mwnt4
Wnts affect ALDH1A2 expression in WiT49 cells. (A) Effects of Wnts on TCF reporter activity in WiT49 cells. 400 ng TOPFlash or FOPFlash constructs with 100 ng <t>pRK5-mWnts</t> expression vectors or pRK-5 empty vector, as well as 25 ng of the pRL-TK renilla control vector, was transiently transfected into WiT49 cells for 48 h. The pRK5-mWnts include pRK5-mWnt1, pRK5-mWnt2b, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt5a, pRK5-mWnt6, pRK5-mWnt7a, pRK5-mWnt7b, pRK5-mWnt9b, and pRK5-mWnt11. Luciferase reporter activities relative to renilla (pRL-TK) are shown. Values are the mean ± SD of four determinations; representative results from at least three separate experiments are shown. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 compared with their respective controls, calculated using the Student’s t -test. (B,C) Effect of Wnts on ALDH1A2 expression in WiT49 cells. WiT49 cells were transiently transfected with pRK5-mWnt1, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt9b, or the empty vector pRK5 for 48 h. The ALDH1A2 expression relative to TBP was measured by real-time RT-PCR (B) . Values are the mean ± SD of three determinations; representative results from at least three separate experiments. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 compared with the empty vector pRK5 control group. The ALDH1A2 protein expression was measured by Western blot (C) . pRM-67-ALDH1A2, positive control of ALDH1A2 protein made by transfection of mammalian expression vector pReceiver-M67-ALDH1A2 to WiT49 cells. pRM-67, negative control of ALDH1A2 protein generated by transfection of pReceiver-M67 empty vector. Per lane, 50 μg of the sample was loaded, and 2 μg ALDH1A2 protein-positive control was loaded. Mock represents samples of cells treated with transfection reagent and without plasmid DNA. Values under bands were calculated by the density of bands of ALDH1A2 divided by the density of bands of GAPDH.
Prk5 Mwnt4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
prk5 mwnt4 - by Bioz Stars, 2026-03
91/100 stars
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prk5 mwnt6  (Addgene inc)
91
Addgene inc prk5 mwnt6
Wnts affect ALDH1A2 expression in WiT49 cells. (A) Effects of Wnts on TCF reporter activity in WiT49 cells. 400 ng TOPFlash or FOPFlash constructs with 100 ng <t>pRK5-mWnts</t> expression vectors or pRK-5 empty vector, as well as 25 ng of the pRL-TK renilla control vector, was transiently transfected into WiT49 cells for 48 h. The pRK5-mWnts include pRK5-mWnt1, pRK5-mWnt2b, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt5a, pRK5-mWnt6, pRK5-mWnt7a, pRK5-mWnt7b, pRK5-mWnt9b, and pRK5-mWnt11. Luciferase reporter activities relative to renilla (pRL-TK) are shown. Values are the mean ± SD of four determinations; representative results from at least three separate experiments are shown. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 compared with their respective controls, calculated using the Student’s t -test. (B,C) Effect of Wnts on ALDH1A2 expression in WiT49 cells. WiT49 cells were transiently transfected with pRK5-mWnt1, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt9b, or the empty vector pRK5 for 48 h. The ALDH1A2 expression relative to TBP was measured by real-time RT-PCR (B) . Values are the mean ± SD of three determinations; representative results from at least three separate experiments. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 compared with the empty vector pRK5 control group. The ALDH1A2 protein expression was measured by Western blot (C) . pRM-67-ALDH1A2, positive control of ALDH1A2 protein made by transfection of mammalian expression vector pReceiver-M67-ALDH1A2 to WiT49 cells. pRM-67, negative control of ALDH1A2 protein generated by transfection of pReceiver-M67 empty vector. Per lane, 50 μg of the sample was loaded, and 2 μg ALDH1A2 protein-positive control was loaded. Mock represents samples of cells treated with transfection reagent and without plasmid DNA. Values under bands were calculated by the density of bands of ALDH1A2 divided by the density of bands of GAPDH.
Prk5 Mwnt6, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
prk5 mwnt6 - by Bioz Stars, 2026-03
91/100 stars
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prk5 mwnt5a  (Addgene inc)
91
Addgene inc prk5 mwnt5a
Wnts affect ALDH1A2 expression in WiT49 cells. (A) Effects of Wnts on TCF reporter activity in WiT49 cells. 400 ng TOPFlash or FOPFlash constructs with 100 ng <t>pRK5-mWnts</t> expression vectors or pRK-5 empty vector, as well as 25 ng of the pRL-TK renilla control vector, was transiently transfected into WiT49 cells for 48 h. The pRK5-mWnts include pRK5-mWnt1, pRK5-mWnt2b, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt5a, pRK5-mWnt6, pRK5-mWnt7a, pRK5-mWnt7b, pRK5-mWnt9b, and pRK5-mWnt11. Luciferase reporter activities relative to renilla (pRL-TK) are shown. Values are the mean ± SD of four determinations; representative results from at least three separate experiments are shown. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 compared with their respective controls, calculated using the Student’s t -test. (B,C) Effect of Wnts on ALDH1A2 expression in WiT49 cells. WiT49 cells were transiently transfected with pRK5-mWnt1, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt9b, or the empty vector pRK5 for 48 h. The ALDH1A2 expression relative to TBP was measured by real-time RT-PCR (B) . Values are the mean ± SD of three determinations; representative results from at least three separate experiments. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 compared with the empty vector pRK5 control group. The ALDH1A2 protein expression was measured by Western blot (C) . pRM-67-ALDH1A2, positive control of ALDH1A2 protein made by transfection of mammalian expression vector pReceiver-M67-ALDH1A2 to WiT49 cells. pRM-67, negative control of ALDH1A2 protein generated by transfection of pReceiver-M67 empty vector. Per lane, 50 μg of the sample was loaded, and 2 μg ALDH1A2 protein-positive control was loaded. Mock represents samples of cells treated with transfection reagent and without plasmid DNA. Values under bands were calculated by the density of bands of ALDH1A2 divided by the density of bands of GAPDH.
Prk5 Mwnt5a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
prk5 mwnt5a - by Bioz Stars, 2026-03
91/100 stars
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prk5 mwnt7a  (Addgene inc)
91
Addgene inc prk5 mwnt7a
Wnts affect ALDH1A2 expression in WiT49 cells. (A) Effects of Wnts on TCF reporter activity in WiT49 cells. 400 ng TOPFlash or FOPFlash constructs with 100 ng <t>pRK5-mWnts</t> expression vectors or pRK-5 empty vector, as well as 25 ng of the pRL-TK renilla control vector, was transiently transfected into WiT49 cells for 48 h. The pRK5-mWnts include pRK5-mWnt1, pRK5-mWnt2b, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt5a, pRK5-mWnt6, pRK5-mWnt7a, pRK5-mWnt7b, pRK5-mWnt9b, and pRK5-mWnt11. Luciferase reporter activities relative to renilla (pRL-TK) are shown. Values are the mean ± SD of four determinations; representative results from at least three separate experiments are shown. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 compared with their respective controls, calculated using the Student’s t -test. (B,C) Effect of Wnts on ALDH1A2 expression in WiT49 cells. WiT49 cells were transiently transfected with pRK5-mWnt1, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt9b, or the empty vector pRK5 for 48 h. The ALDH1A2 expression relative to TBP was measured by real-time RT-PCR (B) . Values are the mean ± SD of three determinations; representative results from at least three separate experiments. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 compared with the empty vector pRK5 control group. The ALDH1A2 protein expression was measured by Western blot (C) . pRM-67-ALDH1A2, positive control of ALDH1A2 protein made by transfection of mammalian expression vector pReceiver-M67-ALDH1A2 to WiT49 cells. pRM-67, negative control of ALDH1A2 protein generated by transfection of pReceiver-M67 empty vector. Per lane, 50 μg of the sample was loaded, and 2 μg ALDH1A2 protein-positive control was loaded. Mock represents samples of cells treated with transfection reagent and without plasmid DNA. Values under bands were calculated by the density of bands of ALDH1A2 divided by the density of bands of GAPDH.
Prk5 Mwnt7a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
prk5 mwnt7a - by Bioz Stars, 2026-03
91/100 stars
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wnts  (Addgene inc)
91
Addgene inc wnts
FIGURE 3 | <t>Wnts</t> affect ALDH1A2 expression in WiT49 cells. (A) Effects of Wnts on TCF reporter activity in WiT49 cells. 400 ng TOPFlash or FOPFlash constructs with 100 <t>ng</t> <t>pRK5-mWnts</t> expression vectors or pRK-5 empty vector, as well as 25 ng of the pRL-TK renilla control vector, was transiently transfected into WiT49 cells for 48 h. The pRK5-mWnts include pRK5-mWnt1, pRK5-mWnt2b, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt5a, pRK5-mWnt6, pRK5-mWnt7a, pRK5-mWnt7b, pRK5-mWnt9b, and pRK5-mWnt11. Luciferase reporter activities relative to renilla (pRL-TK) are shown. Values are the mean ± SD of four determinations; representative results from at least three separate experiments are shown. ∗∗P < 0.01 and ∗∗∗P < 0.001 compared with their respective controls, calculated using the Student’s t-test. (B,C) Effect of Wnts on ALDH1A2 expression in WiT49 cells. WiT49 cells were transiently transfected with pRK5-mWnt1, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt9b, or the empty vector pRK5 for 48 h. The ALDH1A2 expression relative to TBP was measured by real-time RT-PCR (B). Values are the mean ± SD of three determinations; representative results from at least three separate experiments. ∗∗P < 0.01 and ∗∗∗P < 0.001 compared with the empty vector pRK5 control group. The ALDH1A2 protein expression was measured by Western blot (C). pRM-67-ALDH1A2, positive control of ALDH1A2 protein made by transfection of mammalian expression vector pReceiver-M67-ALDH1A2 to WiT49 cells. pRM-67, negative control of ALDH1A2 protein generated by transfection of pReceiver-M67 empty vector. Per lane, 50 µg of the sample was loaded, and 2 µg ALDH1A2 protein-positive control was loaded. Mock represents samples of cells treated with transfection reagent and without plasmid DNA. Values under bands were calculated by the density of bands of ALDH1A2 divided by the density of bands of GAPDH.
Wnts, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
wnts - by Bioz Stars, 2026-03
91/100 stars
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prk5 mwnt9b  (Addgene inc)
91
Addgene inc prk5 mwnt9b
FIGURE 3 | <t>Wnts</t> affect ALDH1A2 expression in WiT49 cells. (A) Effects of Wnts on TCF reporter activity in WiT49 cells. 400 ng TOPFlash or FOPFlash constructs with 100 <t>ng</t> <t>pRK5-mWnts</t> expression vectors or pRK-5 empty vector, as well as 25 ng of the pRL-TK renilla control vector, was transiently transfected into WiT49 cells for 48 h. The pRK5-mWnts include pRK5-mWnt1, pRK5-mWnt2b, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt5a, pRK5-mWnt6, pRK5-mWnt7a, pRK5-mWnt7b, pRK5-mWnt9b, and pRK5-mWnt11. Luciferase reporter activities relative to renilla (pRL-TK) are shown. Values are the mean ± SD of four determinations; representative results from at least three separate experiments are shown. ∗∗P < 0.01 and ∗∗∗P < 0.001 compared with their respective controls, calculated using the Student’s t-test. (B,C) Effect of Wnts on ALDH1A2 expression in WiT49 cells. WiT49 cells were transiently transfected with pRK5-mWnt1, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt9b, or the empty vector pRK5 for 48 h. The ALDH1A2 expression relative to TBP was measured by real-time RT-PCR (B). Values are the mean ± SD of three determinations; representative results from at least three separate experiments. ∗∗P < 0.01 and ∗∗∗P < 0.001 compared with the empty vector pRK5 control group. The ALDH1A2 protein expression was measured by Western blot (C). pRM-67-ALDH1A2, positive control of ALDH1A2 protein made by transfection of mammalian expression vector pReceiver-M67-ALDH1A2 to WiT49 cells. pRM-67, negative control of ALDH1A2 protein generated by transfection of pReceiver-M67 empty vector. Per lane, 50 µg of the sample was loaded, and 2 µg ALDH1A2 protein-positive control was loaded. Mock represents samples of cells treated with transfection reagent and without plasmid DNA. Values under bands were calculated by the density of bands of ALDH1A2 divided by the density of bands of GAPDH.
Prk5 Mwnt9b, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
prk5 mwnt9b - by Bioz Stars, 2026-03
91/100 stars
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Wnts affect ALDH1A2 expression in WiT49 cells. (A) Effects of Wnts on TCF reporter activity in WiT49 cells. 400 ng TOPFlash or FOPFlash constructs with 100 ng pRK5-mWnts expression vectors or pRK-5 empty vector, as well as 25 ng of the pRL-TK renilla control vector, was transiently transfected into WiT49 cells for 48 h. The pRK5-mWnts include pRK5-mWnt1, pRK5-mWnt2b, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt5a, pRK5-mWnt6, pRK5-mWnt7a, pRK5-mWnt7b, pRK5-mWnt9b, and pRK5-mWnt11. Luciferase reporter activities relative to renilla (pRL-TK) are shown. Values are the mean ± SD of four determinations; representative results from at least three separate experiments are shown. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 compared with their respective controls, calculated using the Student’s t -test. (B,C) Effect of Wnts on ALDH1A2 expression in WiT49 cells. WiT49 cells were transiently transfected with pRK5-mWnt1, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt9b, or the empty vector pRK5 for 48 h. The ALDH1A2 expression relative to TBP was measured by real-time RT-PCR (B) . Values are the mean ± SD of three determinations; representative results from at least three separate experiments. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 compared with the empty vector pRK5 control group. The ALDH1A2 protein expression was measured by Western blot (C) . pRM-67-ALDH1A2, positive control of ALDH1A2 protein made by transfection of mammalian expression vector pReceiver-M67-ALDH1A2 to WiT49 cells. pRM-67, negative control of ALDH1A2 protein generated by transfection of pReceiver-M67 empty vector. Per lane, 50 μg of the sample was loaded, and 2 μg ALDH1A2 protein-positive control was loaded. Mock represents samples of cells treated with transfection reagent and without plasmid DNA. Values under bands were calculated by the density of bands of ALDH1A2 divided by the density of bands of GAPDH.

Journal: Frontiers in Cell and Developmental Biology

Article Title: Inhibition of GSK3 Represses the Expression of Retinoic Acid Synthetic Enzyme ALDH1A2 via Wnt/β-Catenin Signaling in WiT49 Cells

doi: 10.3389/fcell.2020.00094

Figure Lengend Snippet: Wnts affect ALDH1A2 expression in WiT49 cells. (A) Effects of Wnts on TCF reporter activity in WiT49 cells. 400 ng TOPFlash or FOPFlash constructs with 100 ng pRK5-mWnts expression vectors or pRK-5 empty vector, as well as 25 ng of the pRL-TK renilla control vector, was transiently transfected into WiT49 cells for 48 h. The pRK5-mWnts include pRK5-mWnt1, pRK5-mWnt2b, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt5a, pRK5-mWnt6, pRK5-mWnt7a, pRK5-mWnt7b, pRK5-mWnt9b, and pRK5-mWnt11. Luciferase reporter activities relative to renilla (pRL-TK) are shown. Values are the mean ± SD of four determinations; representative results from at least three separate experiments are shown. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 compared with their respective controls, calculated using the Student’s t -test. (B,C) Effect of Wnts on ALDH1A2 expression in WiT49 cells. WiT49 cells were transiently transfected with pRK5-mWnt1, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt9b, or the empty vector pRK5 for 48 h. The ALDH1A2 expression relative to TBP was measured by real-time RT-PCR (B) . Values are the mean ± SD of three determinations; representative results from at least three separate experiments. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 compared with the empty vector pRK5 control group. The ALDH1A2 protein expression was measured by Western blot (C) . pRM-67-ALDH1A2, positive control of ALDH1A2 protein made by transfection of mammalian expression vector pReceiver-M67-ALDH1A2 to WiT49 cells. pRM-67, negative control of ALDH1A2 protein generated by transfection of pReceiver-M67 empty vector. Per lane, 50 μg of the sample was loaded, and 2 μg ALDH1A2 protein-positive control was loaded. Mock represents samples of cells treated with transfection reagent and without plasmid DNA. Values under bands were calculated by the density of bands of ALDH1A2 divided by the density of bands of GAPDH.

Article Snippet: Expression plasmids of multiple Wnts, including pRK5-mWnt1 (Addgene #42273), pRK5-mWnt2b (Addgene #42275), pRK5-mWnt3a (Addgene #42277), pRK5-mWnt4 (Addgene #42278), pRK5-mWnt5a (Addgene #42279), pRK5-mWnt6 (Addgene #42281), pRK5-mWnt7a (Addgene #42282), pRK5-mWnt7b (Addgene #42283), pRK5-mWnt9b (Addgene #42287) and pRK5-mWnt11 (Addgene #42290), were gifts from Chris Garcia and Jeremy Nathans ( ). pRK5 empty vector was made by re-ligation of the backbone of pRK5-mWnt4 (Addgene #42278) digested by Pst I. ALDH1A2 promoter or intron1G was cloned using the primers shown in .

Techniques: Expressing, Activity Assay, Construct, Plasmid Preparation, Transfection, Luciferase, Quantitative RT-PCR, Western Blot, Positive Control, Negative Control, Generated

FIGURE 3 | Wnts affect ALDH1A2 expression in WiT49 cells. (A) Effects of Wnts on TCF reporter activity in WiT49 cells. 400 ng TOPFlash or FOPFlash constructs with 100 ng pRK5-mWnts expression vectors or pRK-5 empty vector, as well as 25 ng of the pRL-TK renilla control vector, was transiently transfected into WiT49 cells for 48 h. The pRK5-mWnts include pRK5-mWnt1, pRK5-mWnt2b, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt5a, pRK5-mWnt6, pRK5-mWnt7a, pRK5-mWnt7b, pRK5-mWnt9b, and pRK5-mWnt11. Luciferase reporter activities relative to renilla (pRL-TK) are shown. Values are the mean ± SD of four determinations; representative results from at least three separate experiments are shown. ∗∗P < 0.01 and ∗∗∗P < 0.001 compared with their respective controls, calculated using the Student’s t-test. (B,C) Effect of Wnts on ALDH1A2 expression in WiT49 cells. WiT49 cells were transiently transfected with pRK5-mWnt1, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt9b, or the empty vector pRK5 for 48 h. The ALDH1A2 expression relative to TBP was measured by real-time RT-PCR (B). Values are the mean ± SD of three determinations; representative results from at least three separate experiments. ∗∗P < 0.01 and ∗∗∗P < 0.001 compared with the empty vector pRK5 control group. The ALDH1A2 protein expression was measured by Western blot (C). pRM-67-ALDH1A2, positive control of ALDH1A2 protein made by transfection of mammalian expression vector pReceiver-M67-ALDH1A2 to WiT49 cells. pRM-67, negative control of ALDH1A2 protein generated by transfection of pReceiver-M67 empty vector. Per lane, 50 µg of the sample was loaded, and 2 µg ALDH1A2 protein-positive control was loaded. Mock represents samples of cells treated with transfection reagent and without plasmid DNA. Values under bands were calculated by the density of bands of ALDH1A2 divided by the density of bands of GAPDH.

Journal: Frontiers in cell and developmental biology

Article Title: Inhibition of GSK3 Represses the Expression of Retinoic Acid Synthetic Enzyme ALDH1A2 via Wnt/β-Catenin Signaling in WiT49 Cells.

doi: 10.3389/fcell.2020.00094

Figure Lengend Snippet: FIGURE 3 | Wnts affect ALDH1A2 expression in WiT49 cells. (A) Effects of Wnts on TCF reporter activity in WiT49 cells. 400 ng TOPFlash or FOPFlash constructs with 100 ng pRK5-mWnts expression vectors or pRK-5 empty vector, as well as 25 ng of the pRL-TK renilla control vector, was transiently transfected into WiT49 cells for 48 h. The pRK5-mWnts include pRK5-mWnt1, pRK5-mWnt2b, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt5a, pRK5-mWnt6, pRK5-mWnt7a, pRK5-mWnt7b, pRK5-mWnt9b, and pRK5-mWnt11. Luciferase reporter activities relative to renilla (pRL-TK) are shown. Values are the mean ± SD of four determinations; representative results from at least three separate experiments are shown. ∗∗P < 0.01 and ∗∗∗P < 0.001 compared with their respective controls, calculated using the Student’s t-test. (B,C) Effect of Wnts on ALDH1A2 expression in WiT49 cells. WiT49 cells were transiently transfected with pRK5-mWnt1, pRK5-mWnt3a, pRK5-mWnt4, pRK5-mWnt9b, or the empty vector pRK5 for 48 h. The ALDH1A2 expression relative to TBP was measured by real-time RT-PCR (B). Values are the mean ± SD of three determinations; representative results from at least three separate experiments. ∗∗P < 0.01 and ∗∗∗P < 0.001 compared with the empty vector pRK5 control group. The ALDH1A2 protein expression was measured by Western blot (C). pRM-67-ALDH1A2, positive control of ALDH1A2 protein made by transfection of mammalian expression vector pReceiver-M67-ALDH1A2 to WiT49 cells. pRM-67, negative control of ALDH1A2 protein generated by transfection of pReceiver-M67 empty vector. Per lane, 50 µg of the sample was loaded, and 2 µg ALDH1A2 protein-positive control was loaded. Mock represents samples of cells treated with transfection reagent and without plasmid DNA. Values under bands were calculated by the density of bands of ALDH1A2 divided by the density of bands of GAPDH.

Article Snippet: Expression plasmids of multiple Wnts, including pRK5mWnt1 (Addgene #42273), pRK5-mWnt2b (Addgene #42275), pRK5-mWnt3a (Addgene #42277), pRK5-mWnt4 (Addgene #42278), pRK5-mWnt5a (Addgene #42279), pRK5-mWnt6 (Addgene #42281), pRK5-mWnt7a (Addgene #42282), pRK5mWnt7b (Addgene #42283), pRK5-mWnt9b (Addgene #42287) and pRK5-mWnt11 (Addgene #42290), were gifts from Chris Garcia and Jeremy Nathans (Yu et al., 2012). pRK5 empty vector was made by re-ligation of the backbone of pRK5-mWnt4 (Addgene #42278) digested by PstI.

Techniques: Expressing, Activity Assay, Construct, Plasmid Preparation, Control, Transfection, Luciferase, Quantitative RT-PCR, Western Blot, Positive Control, Negative Control, Generated